SOFT/TIAFT 98 - Detection Times of 6-Acetylmorphine, Free Morphine and...

SOFT - TIAFT 1998

Scientific Session 2

Wednesday October 7, 1998

DETECTION TIMES OF 6-ACETYLMORPHINE, FREE MORPHINE AND TOTAL MORPHINE IN URINE AFTER SMOKING HEROIN
	Michael L. Smith, Budda D. Paul, Eric Shimomura, Jacqueline Summers, Amanda J. Jenkins, William D. Darwin and Edward J. Cone* Division of Forensic Toxicology, Armed Forces Iinstitute of Pathology, Rockville, MD, USA. * Addiction Research Center, IRP, NIDA, NIH, Baltimore, MD, USA

Heroin was administered to four human subjects at doses of 3.5-13.9 mg by smoking. Urine samples were collected and tested by GC-MS procedures under blind conditions. The total amounts of 6-AM, free morphine and total morphine (M ± SEM) excreted in urine were 0.49 ± 0.14, 4.6 ± 1.0, and 53.5 ± 10.8 mole percent of the administered heroin, respectively. Detection times of the last specimen positive for the heroin metabolites at the cutoff concentrations indicated are presented in the table.

Heroin	6-AM	Free Morphine		Total Morphine
Dose, mg	10 ng/mL	100 ng/mL	300 ng/mL	2000 ng/mL	4000 ng/mL

3.5 (n = 4)	0-4.9 hr	2.3-5.7 hr	7.9-27.5 hr	0-4.9 hr	0-2.3 hr
5.2 (n = 1)	2.3	2.9	7.5	2.3	0
7.0 (n = 2)	0-2.4	2.2-2.4	10.0-16.4	2.4-6.0	0
10.5 (n = 2)	2.3-5.9	2.3-8.4	10.7-28.9	2.3-8.4	0-5.9
13.9 (n = 1)	11.2	22.3	47.8	22.3	11.2

Free morphine and total morphine at cutoff concentrations of 100 ng/mL and 2000 ng/mL, respectively, correlated better with 6-AM at a cutoff concentration of 10 ng/mL and produced fewer false positives and false negatives than total morphine at the cutoff concentrations of 300 or 4000 ng/mL. Measurement of total morphine and 6-AM required two confirmation methods. Measurement of free morphine had the advantage that it could be assayed with 6-AM in one confirmation procedure.

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