Department of Pathology, Medical College of Virginia Campus at Virginia Commonwealth University, Richmond, VA. 23298-0165, USA

Calibration curves were linear from 50 to 5,000 ng/mL with a LOD of 20 ng/mL and a LOQ of 50 ng/mL. The overall within run precision of the method yielded CVs of 6% (d-amphetamine), 8% (l-amphetamine), 4% (d-methamphetamine), and 6% (l-methamphetamine) at 500 ng/mL (n=5) and 2% (d-amphetamine), 5% (l-amphetamine, 3% (d-methamphetamine), and 3% (l-methamphetamine) at 2500 ng/mL (n = 5). The overall recovery of the method was 97% at analyte concentrations of 500 ng/mL and 102% at analyte concentrations of 2500 ng/mL. Popular phenylisopropylamine drugs were found not to interfere with the method. The results of enantiomeric analysis of 30 urines from methamphetamine abusers by the presented method and chiral GC/MS were compared by linear regression analysis which yielded good correlation for each enantiomer: r² s ranged from 0.952 to 0.974. An advantage of the chiral HPLC method over the chiral GC/MS method was the greater accuracy in determining the ratio of the amphetamine optical isomers as there was no contribution to apparent isomer content due to contamination of d-TPC in l-TPC GC/MS derivatizing reagent.