Republic Scientific Research Crime Center, Tashkent, Uzbekistan

For this purpose, it is necessary to collect in a micro syringe 1 µL acetyl anhydride or trimethylchlorsilane (TMS), then 2 µL 0.2 % alcohol solutions of the drug or of an extract from the biological material. This mixture is injected to the chromatograph evaporation device ("Hewlett-Packard" - HP5890 with FID, glass column 2 m x 3 mm with 5 % SE - 30 on Inerton AW, injection port temperature 270°C). Thus, paracetamol at 200°C column temperature in the presence of acetyl anhydride completely derivatizes; at 235°C tazepam will form acetyltazepam with 100% efficiency, and clopheline is only 28% converted to acetylclopheline. With trimethylchlorsilane phencarolum is completely converted to the trimethilsilyl derivative. Use of these micro chemical reactions in forensic - toxicological analysis has allowed skipping a stage of extraction in preparations from biological materials, and detection of the presence of tazepam and clopheline in stomach contents. A small quantity phencarolum (up to 1 mg) was detected in the liver of a corpse.

The method of direct reaction chromatography may be applied to determination of the nature of medicinal materials in a variety of biological materials, especially if the small quantity of substance present does not allow determination by other methods.